Plan The initial variable to be considered will be the effect that caffeine and temperature has on the heart beat and if it has a direct or indirect effect on the daphnia's lifespan. If there is enough time to consider a second variable however, the effects of a temperature or alcohol on the daphnia's body system will also be considered alongside the initial variable of caffeine. These will then be slotted into the same graph …showed first 75 words of 4752 total…

…showed last 75 words of 4752 total…one minute) 0% 187 184 189 20% 179 184 182 40% 154 157 149 50% 143 144 139 60% 137 131 142 70% 127 125 124 80% 121 116 101 90% 117 84 89 100% dead dead dead Preliminary caffeine exp. Percentage of caffeine in water solution Test 1(amount of beats in one minute) Test 2(amount of beats in one minute) Test 3(amount of beats in one minute) 0% 207 207 207 20% 244 247 241 40% 300 309 307 60% 333 339 345 80% toomany tocount 347 349 100% dead dead dead Actual caffeine exp. Percentage of caffeine in water solution Test 1(amount of beats in one minute) Test 2(amount of beats in one minute) Test 3(amount of beats in one minute) 0% 204 211 209 20% 247 249 254 40% 299 304 311 50% 310 316 319 60% 334 339 347 70% 344 340 339 80% 341 344 347 90% 301 dead dead 100% dead dead dead